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Fitopatologia Brasileira

Print version ISSN 0100-4158On-line version ISSN 1678-4677


MEINHARDT, LYNDEL W.; WULFF, NELSON A.; BELLATO, CLÁUDIA M.  and  TSAI, SIU M.. Genetic analyses of Rhizoctonia solani isolates from Phaseolus vulgaris grown in the Atlantic Rainforest Region of São Paulo, Brazil. Fitopatol. bras. [online]. 2002, vol.27, n.3, pp.259-267. ISSN 0100-4158.

Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris) grown in the mountainous Atlantic Rainforest (Mata Atlântica) region of São Paulo, Brazil, were analyzed to determine their genetic diversity using internal transcribed spacer (ITS), microsatellite and telomere sequence-based PCR primers. Restriction digestion of the ITS1/5.8S/ITS2 ribosomal regions yielded unique banding patterns specific for AG4 and its subgroups. The ITS restriction digestion (ITS/RFLP), telomere and microsatellite primers identified five to 11 genotypes within the isolates of R. solani. While all isolates were pathogenic on beans, there was no correlation found between genotypic differences and pathogenicity. The different PCR primers revealed a number of isolates that were genetically similar. Some of these genetic groups were supported by more than one of the primers utilized in this study, thus confirming their relationship.

Keywords : Mata Atlântica; genetic biodiversity; soil-borne fungi; ITS; telomere.

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