versão impressa ISSN 0100-4158
ROCHA, Fernando S. e CAMPOS, Vicente P.. Effect of exudates of plant cell culture on second-stage juveniles of Meloidogyne incognita. Fitopatol. bras. [online]. 2004, vol.29, n.3, pp. 294-299. ISSN 0100-4158. http://dx.doi.org/10.1590/S0100-41582004000300009.
Callus of Lycopersicon esculentum, Coffea arabica, Medicago sativa, Dendrobium nobile, Brassica rapa, Ipomoea batatas, Nicotiana tabacum, Daucus carota and Crotalaria juncea were obtained in Murashige & Skoog (MS) solid medium followed by cell cultivation in MS liquid medium at temperature varying from 25-28 ºC. After one month, the cell suspension was passed through a 0,22 µm Millipore membrane, and the resulting liquid was the cell exudate from each of the tested plants. Eggs or second-stage juveniles (J2) of Meloidogyne incognita were then incubated in these exudates and hatching, mobility and mortality percentages of the J2 were evaluated. Except for the eggs incubated in the exudate of orchid, all the exudates inhibited J2 hatching when compared with incubation in water (control). However, in L. esculentum, C. arabica and C. juncea exudates the inhibition was greatest, similar to aldicar, but significantly less and different than that found in solution of culture medium ingredients MS (1-5). All exudates reduced mobility and increased mortality of M. incognita J2, although theses were enhanced when exposed for 24 h. The greatest reduction of mobility occurred with the exudates of tomato and M. sativa, while greatest mortality occurred with the exudate of L. esculentum, followed by B. rapa.
Palavras-chave : calus; tomate; mostarda; alfafa; cenoura; café; Crotalaria juncea; fumo; batata doce; orquídea; nematóide das galhas; substâncias tóxicas; exsudato.