SciELO - Scientific Electronic Library Online

 
vol.30 issue4Evidence of induction of systemic resistance to eucalyptus rust by plant growth promoting rhizobacteriaBiological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Fitopatologia Brasileira

Print version ISSN 0100-4158

Abstract

SOUZA JUNIOR, Manoel T.; NICKEL, Osmar  and  GONSALVES, Dennis. Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus. Fitopatol. bras. [online]. 2005, vol.30, n.4, pp. 357-365. ISSN 0100-4158.  http://dx.doi.org/10.1590/S0100-41582005000400004.

Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2 populations were incorporated into the papaya-breeding program at Embrapa Cassava and Tropical Fruits, in Cruz das Almas, Bahia, Brazil.

Keywords : Carica papaya; potyvirus; resistance; breeding; virus.

        · abstract in Portuguese     · text in English     · pdf in English