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SARTORATO, Aloísio; NECHET, Kátia L. and HALFELD-VIEIRA, Bernardo A.. Genetic diversity of Rhizoctonia solani isolates collected from Cowpea in the State of Roraima. Fitopatol. bras. [online]. 2006, vol.31, n.3, pp. 297-301. ISSN 0100-4158. http://dx.doi.org/10.1590/S0100-41582006000300009.
Cowpea (Vigna unguiculata) is one of the most important sources of protein for people with low incomes in Northern and Northeastern Brazil. Cowpea is susceptible to several diseases, including web blight caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris). Although R. solani is a very important disease agent, in Brazil there is no information on isolate characteristics when this fungus is associated with cowpea. The objective of this study was to evaluate, using Random Amplified Polymorphic DNA (RAPD) and RFLP-ITS (Internal Transcribed Spacer) techniques, the genetic diversity of R. solani isolates collected in the State of Roraima from cowpea cultivated under 'savannah' and 'forest' ecosystems. From the results it was possible to demonstrate genetic diversity among R. solani isolates and to prove that both molecular techniques used were efficient in evaluating it.
Keywords : RAPD; ITS; Vigna unguiculata; web blight.