SciELO - Scientific Electronic Library Online

vol.34 issue2Flow injection visible diffuse reflectance quantitative analysis of total sulfur in biodiesel, in plant leaves and in natural watersSynthesis of long chain ester by heterogeneous base catalysis author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Eclética Química

Print version ISSN 0100-4670


VISANI, Valeria et al. Flow-batch analyser for preparation of calibration standard mixtures in simultaneous multicomponent spectrometric analysis. Eclet. Quím. [online]. 2009, vol.34, n.2, pp.37-47. ISSN 0100-4670.

The application of multivariate calibration techniques to multicomponent analysis by UV-VIS molecular absorption spectrometry is a powerful tool for simultaneous determination of several chemical species. However, when this methodology is accomplished manually, it is slow and laborious, consumes high amounts of reagents and samples, is susceptible to contaminations and presents a high operational cost. To overcome these drawbacks, a flow-batch analyser is proposed in this work. This analyser was developed for automatic preparation of standard calibration and test (or validation) mixtures. It was applied to the simultaneous determination of Cu2+, Mn2+ and Zn2+ in polyvitaminic and polymineral pharmaceutical formulations, using 4-(2-piridilazo) resorcinol as reagent and a UV-VIS spectrophotometer with a photodiode array detector. The results obtained with the proposed system are in good agreement with those obtained by flame atomic absorption spectrometry, which was employed as reference method. With the proposed analyser, the preparation of calibration and test mixtures can be accomplished about four hours, while the manual procedure requires at least two days. Moreover, it consumes smaller amounts of reagents and samples than the manual procedure. After the preparation of calibration and test mixtures, 60 samples h-1 can be carried out with the proposed flow-batch analyser.

Keywords : Flow-batch analyser; preparation of standard mixtures; simultaneous multicomponent analysis; UV-VIS spectrophotometry; drug analysis.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License