Abstract

SILVA, Vitor Paulo Andrade da et al. Biossensor amperométrico para determinação de peróxido de hidrogênio em leite. Eclet. Quím. [online]. 2011, vol.36, n.2, pp. 143-157. ISSN 0100-4670.  http://dx.doi.org/10.1590/S0100-46702011000200008.

An amperometric biosensor was developed for detection of hydrogen peroxide in milk samples. The biosensor was constructed from the immobilization of peroxidase on printed carbon electrode. Optimization parameters were evaluated in order to obtain better performance of the biosensor. The biosensor showed linearity in the range 5.0 to 40.0 µ mol L^-1H₂O₂ in phosphate buffer. Milk samples without dilution reported the detection limit for H₂O₂ of 0.42 µmol L^-1 and quantification of 1.39 µmol L^-1.The biosensor could be a sensitive and inexpensive alternative for the detection of H₂O₂ in adulterated milk samples.

Keywords : Peroxidase radish; screen printed; glutaraldehyde; biosensor.