Print version ISSN 0100-5405
On-line version ISSN 1980-5454
KRAUSE-SAKATE, Renate et al. Quantitative control of Lettuce mosaic virus fitness and host defence inhibition by P1-HCPro. Summa phytopathol. [online]. 2007, vol.33, n.2, pp.119-123. ISSN 0100-5405. http://dx.doi.org/10.1590/S0100-54052007000200003.
Two Lettuce mosaic virus isolates capable of overcoming the resistance afforded by the resistance gene mo12 in lettuce, LMV-AF199 from Brazil, and LMV-E, an European isolate, were evaluated for the rapidity and severity of symptoms induced on the lettuce variety Salinas 88 (mo12). The mosaic symptoms on Salinas 88 plants inoculated with LMV-AF199 appeared 7 days post-inoculation (dpi) and 15 dpi for LMV-E. The symptoms induced by LMV-AF199 in this cultivar were also more severe than those induced by LMV-E. In order to identify the region of the viral genome responsible for this phenotype, recombinant viruses were constructed between these isolates and the phenotype of each recombinant was analysed. The region encoding proteins P1 and HcPro from LMV-AF199 was associated with the increased virulence in Salinas 88.
Keywords : Gene silencing; Lactuca sativa; infectious cDNA; Potyvirus.