SciELO - Scientific Electronic Library Online

 
vol.33 número3Identidade molecular dos fitoplasmas associados aos enfezamentos do tomateiro e da berinjela com base na análise do gene 16S rDNAInfectividade de juvenis do segundo estádio de Meloidogyne incognita em tomateiro após privação alimentar em solo e água em diferentes condições índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Summa Phytopathologica

versão impressa ISSN 0100-5405versão On-line ISSN 1980-5454

Resumo

FERREIRA, Paulo de Tarso Oliveira et al. Characterization of a bacilliform virus isolated from Solanum violaefolium transmitted by the tenuipalpid mites Brevipalpus phoenicis and Brevipalpus obovatus. Summa phytopathol. [online]. 2007, vol.33, n.3, pp.264-269. ISSN 0100-5405.  https://doi.org/10.1590/S0100-54052007000300009.

Solanum violaefolium is an ornamental plant, with prostrate, trailing growth habit and is cultivated in shaded areas. A virus that causes ringspot symptoms on its leaves, tentatively named as Solanum violaefolium ringspot virus (SvRSV) and transmitted by Brevipalpus phoenicis (Acari: Tenuipalpidae) was found in Piracicaba city, São Paulo State. It is a bacilliform virus that resembles other cytoplasmic viruses transmitted by Brevipalpus sp. The objective of this work is to describe the biological properties and establish partial molecular characterization of the SvRSV. The SvRSV can be transmitted mechanically to several plant species causing local lesions. Among the tested species, Datura stramonium was proved to be the best experimental host. It was observed that S. violaefolium plants were infested by B. obovatus that also transmitted SvRSV in preliminary assays. Its in vitro physical properties were: thermal inactivation point: 40-45 ºC; dilution end point: 10-3-10-4 and in vitro longevity: 12 days. Ultrathin sections revealed that SvRSV particles are slightly thinner and longer than other cytoplasmic-type viruses transmitted by Brevipalpus sp. A cDNA library from dsRNA molecules extracted from SvRSV was constructed and two regions, which putatively code for the replicase and movement proteins were identified. Based on these sequences, primer pairs were designed for SvRSV´s RNA amplification by RT-PCR. Indeed, probes based on such sequences hybridized with ss- and dsRNA from infected D. stramonium plants. Preliminary molecular assays using primers and probes of SvRSV did not detect Citrus leprosis virus, cytoplasmic type (CiLV-C), another cytoplasmic type Brevipalpus-transmitted viruses.

Palavras-chave : SvRSV; electron microscopy; dsRNA; sequencing; cytopatic effect; CiLV-C.

        · resumo em Português     · texto em Português     · Português ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons