Print version ISSN 0100-5405
SILVEIRA, Daniela Garcia et al. Germplasm biological indexing for Banana streak virus. Summa phytopathol. [online]. 2008, vol.34, n.2, pp. 172-174. ISSN 0100-5405. http://dx.doi.org/10.1590/S0100-54052008000200012.
The Germplasm Active Bank (BAG) of banana is the base of the genetic breeding program of Embrapa Cassava and Tropical Fruits. The objective of this work was to index the accessions of the BAG for Banana streak virus (BSV). Each sample collected in the 220 accessions of BAG was used to inoculate three 'Caipira' banana plants, produced by micropropagation. The plants were inoculated using the mealybug Planococcus citri Risso as vector. The vector being allowed an access of acquisition of 24 hours and 48 hours of transmission. Plants were previously analysed by PCR for the presence or absence of BSV were used as positive and negative control, respectively. Between 15 and 70 days after inoculation the test plants showed the first disease symptoms. Using this methodology, it was observed that 44 of the 220 accesses were infected with BSV.
Keywords : BSV; Musa spp.; Active Germplasm Bank.