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On-line version ISSN 1806-9088
CARVALHO, Jane Fiuza Rodrigues Portela de; CARVALHO, Carlos Roberto de and OTONI, Wagner Campos. In vitro regeneration of annatto (Bixa orellana L.) from various explants. Rev. Árvore [online]. 2005, vol.29, n.6, pp.887-895. ISSN 1806-9088. http://dx.doi.org/10.1590/S0100-67622005000600007.
The present work aimed the establishment of a regeneration protocol for annatto (Bixa orellana L.) from different juvenile explants. In order to promote shoot induction, different concentrations and/or combinations of IAA and the cytokinins BAP and ZEA were assessed. Better regeneration responses were achieved when segmented hypocotyl, cotiledonary nodes and inverted hypocotyl were cultured onto MS-based medium supplemented with ZEA (2.28 µM) and IAA (0.30 µM), ZEA (4.56 µM) or ZEA (4.56 µM), respectively. Rooting of elongated shoots displayed higher frequencies when half-strength MS medium with IBA (5 µM) was used. No genetic variation was detected among regenerants as revealed by cytological analysis based on metaphasic chromosome countings (2n = 14).
Keywords : micropropagation; organogenesis; annatto; tissue culture.