SciELO - Scientific Electronic Library Online

vol.29 issue6Influence of size and provenance on the germination and vigor of Mimosa caesalpiniifolia Benth. SeedsEffect of nursery substrata and containers on root system architecture of black wattles trees author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Árvore

On-line version ISSN 1806-9088


CARVALHO, Jane Fiuza Rodrigues Portela de; CARVALHO, Carlos Roberto de  and  OTONI, Wagner Campos. In vitro regeneration of annatto (Bixa orellana L.) from various explants. Rev. Árvore [online]. 2005, vol.29, n.6, pp.887-895. ISSN 1806-9088.

The present work aimed the establishment of a regeneration protocol for annatto (Bixa orellana L.) from different juvenile explants. In order to promote shoot induction, different concentrations and/or combinations of IAA and the cytokinins BAP and ZEA were assessed. Better regeneration responses were achieved when segmented hypocotyl, cotiledonary nodes and inverted hypocotyl were cultured onto MS-based medium supplemented with ZEA (2.28 µM) and IAA (0.30 µM), ZEA (4.56 µM) or ZEA (4.56 µM), respectively. Rooting of elongated shoots displayed higher frequencies when half-strength MS medium with IBA (5 µM) was used. No genetic variation was detected among regenerants as revealed by cytological analysis based on metaphasic chromosome countings (2n = 14).

Keywords : micropropagation; organogenesis; annatto; tissue culture.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License