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vol.30 issue1Alternative to attenuate plant stand difference in experiments of Eucalyptus urophylla clone assessmentMorphological characterization, viability and vigor of Tabebuia aurea (Silva Manso) Benth. & Hook. f. ex. S. Moore seeds author indexsubject indexarticles search
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Revista Árvore

Print version ISSN 0100-6762On-line version ISSN 1806-9088

Abstract

CARVALHO, Dulcinéia de et al. Proteins and isozymes electroforesis in seeds of Copaifera Langsdorffii Desf. (leguminosae caesalpinioideae) artificially aged. Rev. Árvore [online]. 2006, vol.30, n.1, pp.19-24. ISSN 0100-6762.  https://doi.org/10.1590/S0100-67622006000100003.

Electrophoresis has been used to aid the evaluation of physiological seed quality of forest species, as isozymes provide fast responses. Seeds of copaiba (Copaifera langsdorffii) were artificially aged for 0, 24, 48, 72 and 96 hours, in a germination chamber (42º C and 100% UR). The objective of this research was to evaluate possible changes in the electrophoretic patterns of proteins and isozymes during the artificial aging. The artificial aging reduced the germination and vigor of C. langsdorffii seeds, but vigor was affected faster than germination. The electrophoretic patterns showed no difference in the content of soluble and storage proteins, but for esterase and peroxidase isozymes there was reduction in the activity with aging.

Keywords : Seed germination; artificial aging; electrophoresis; Copaifera langsdorffii.

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