On-line version ISSN 1806-9088
OLIVEIRA, Mila Liparize de et al. Effects of culture medium and BAP/NAA ratio on in vitro multiplication of Eucalyptus grandis x E. urophylla clones in a temporary immersion bioreactor. Rev. Árvore [online]. 2011, vol.35, n.6, pp.1207-1217. ISSN 1806-9088. http://dx.doi.org/10.1590/S0100-67622011000700007.
Three individual experiments were conducted in order to test different culture media and BAP/NAA combinations on the multiplication of Eucalyptus grandis x E. urophylla clones using the temporary immersion bioreactor RITA®. The MS medium and the frequency of immersion every two hours promoted the highest fresh weight and number of shoots per explant. However, cultures growth were different between the two clones evaluated. The combination of 1.0 µM BAP and 0.5 µM NAA promoted the greatest means in relation to fresh weight and number of shoots. The cultures presented high percentages of hyperhydricity and this physiological disorder was a limiting factor in the condition of this study Eucalyptus cultivation in bioreactors.
Keywords : Micropropagation; Cloning of eucalyptus; Biotechnology.