EVALUATION OF SUBSTRATES AND AMF SPORULATION IN THE PRODUCTION OF SEEDLINGS OF NATIVE FOREST SPECIES

Gonzaga, Luciana de Moura; Silva, Sarah Santos da; Campos, Silvane de Almeida; Ferreira, Rodrigo de Paula; Campos, André Narvaes da Rocha; Cunha, Ana Catarina Monteiro Carvalho Mori da

doi:10.1590/0100-67622016000200007

Luciana de Moura Gonzaga

Universidade Federal de Lavras, Programa de Pós-Graduação em Engenharia Florestal, Lavras, MG, Brasil. E-mail: <lucianagonzaga11@hotmail.com>.

Sarah Santos da Silva

Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de Minas Gerais, Campus Rio Pomba, Graduanda em Agroecologia, Rio Pomba, MG, Brasil. E-mail: <sarahs2209@hotmail.com>.

Silvane de Almeida Campos

Universidade Federal de Viçosa, Programa de Pós-Graduação em Agroecologia, Viçosa, MG, Brasil. E-mail: <silvaneacampos@yahoo.com.br>.

Rodrigo de Paula Ferreira

Consorcio de Empregadores Cecilia M S. Mattos e Outros, Fazenda Braúna, Ervália, MG, Brasil. E-mail: <rodrigo_lenny@yahoo.com.br>.

André Narvaes da Rocha Campos

Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de MG, Campus Rio Pomba, Departamento Acadêmico de Agricultura e Ambiente, Rio Pomba, MG - Brasil. E-mail: <andre.campos@ifsudestemg.edu.br> and <catarina.mori@ifsudestemg.edu.br>.

Ana Catarina Monteiro Carvalho Mori da Cunha

Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de MG, Campus Rio Pomba, Departamento Acadêmico de Agricultura e Ambiente, Rio Pomba, MG - Brasil. E-mail: <andre.campos@ifsudestemg.edu.br> and <catarina.mori@ifsudestemg.edu.br>.

*Corresponding author.

Table 1 Chemical analysis of samples of soil, sand and organic material (poultry and bovine manures) used in the production of seedlings before treatment application. Held in the Laboratório de Análises de Solos Viçosa Ltda (Viçosa Laboratory of Soil Analysis), Viçosa. - MG

SAMPLES	pH	P	K	Ca²⁺	Mg²⁺	Al ³⁺	H + Al		SB	CTC (t)	CTC (T)	V	M	P-rem
SAMPLES	H₂O	mg/dm³					cmol_c/dm³					%		mg/L
Sand	5,7	3,3	16	0,2	0,1	0	0,17		0,34	0,34	0,51	67	0	47
Soil	4,9	3,8	44	0,5	0,1	0,5	2,97		0,71	1,21	3,68	19	41	11,7
ORGANIC	N	P	K	Ca	Mg	S	CO	C/N	Zn	Fe	Mn Cu	B	pH	Na
MATERIAL	^_____%^_____						(%)		^______ppm^______				H₂O	(%)
PM	2,09	1,72	0,80	2,22	0,32	0,36	33,07	15,80	160	1335	144 37	23,4	6,4	0,176
BM	0,89	0,55	0,32	0,99	0,22	0,46	8,89	9,98	125	23670	430 56	16,4	6,6	0,023

Table 2 Shoot (SDM), root (RDM) and total (TDM) dry matter of seedlings of canafistula (Peltophorum dubium) (Spreng.)Taub, cutieira (Joannesia princeps Vell.), rubber tree (Hevea brasiliensis M. Arg.) at the end of 180 days and of jatoba (Hymenaea courbaril L.) at the end of 210 days

FV	DF	CANAFIS TULA
FV	DF	SDM (g)	RDM (g)	TDM (g)
Treatments	9	6,51 ^ Significant at level of 1% probability (p < .01)	4,52 ^ Significant at level of 1% probability (p < .01)	6,36 ^ Significant at level of 1% probability (p < .01)
CV (%)	9	38,94	71,74	44,64
FV	DF	CUTIEIRA
FV	DF	SDM (g)	RDM (g)	TDM (g)
Treatments	9	1,48 ^ns ns Non significant (p >= .05). F Values obtained.	1,19 ^ns ns Non significant (p >= .05). F Values obtained.	1,43 ^ns ns Non significant (p >= .05). F Values obtained.
CV (%)	9	49,86	35,21	42,54
FV	DF	JATOBA
FV	DF	SDM (g)	RDM (g)	TDM (g)
Treatments	9	0,99 ^ns ns Non significant (p >= .05). F Values obtained.	1,02 ^ns ns Non significant (p >= .05). F Values obtained.	1,04 ^ns ns Non significant (p >= .05). F Values obtained.
CV (%)	9	38,41	44,83	38,5
FV	DF	RUBBER TREE
FV	DF	SDM (g)	RDM (g)	TDM (g)
Treatments	9	1,12 ^ns ns Non significant (p >= .05). F Values obtained.	1,8 ^ns ns Non significant (p >= .05). F Values obtained.	1,2 ^ns ns Non significant (p >= .05). F Values obtained.
CV (%)	9	25,27	23,15	21,46

Table 3 Shoot (SDM), root (RDM) and total (TDM) dry matter of seedlings of canafistula (Peltophorum dubium) (Spreng.) Taub. at the end of 180 days

SUBSTRATES	CANAFISTULA
SUBSTRATES	SDM (g)	RDM (g)	TDM (g)
T1 - So	1,26 b	0,29 b	1,55 c
T2 - So+CF	1,30 b	0,33 b	1,63 c
T3: 80%So:20%Sa	1,69 b	0,55 b	2,24 c
T4: 80% So:20%Sa+CF	1,50 b	0,29 b	1,79 c
T5: 60%So:20%Sa: 20%PM	2,80 b	1,44 a	4,24 b
T6: 50%So:20%Sa:30%PM	4,82 a	2,03 a	6,85 a
T7: 40%So:20%Sa:40%PM	3,43 a	2,96 a	6,39 a
T8: 60%So:20%Sa:20%BM	2,03 b	0,72 b	2,76 c
T9: 50%So:20%Sa:30%BM	4,45 a	2,21 a	6,66 a
T10: 40%So:20%Sa:40%BM	2,63 b	1,57 a	4,20 b

Table 4 Effect of substrates on the growth of seedlings of canafístula (Peltophorum dubium) (Spreng.) Taub, cutieira (Joannesia princeps Vell.) and rubber tree (Hevea brasiliensis M. Arg.) at the end of 180 days and Jatoba (Hymenaea courbaril L.) at the end of 210 days for the production of spores (SPORES) and the ratio spores per root dry matter (SPORES/RDM)

FV	DF	SPORES	SPORES/RDM
Substrates (S)	9	19,25 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.	8,8 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.
Species (Sp)	3	11,41 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.	25,24 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.
S X Sp	27	4,3 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.	3,32 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.
Treatments	39	8,3 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.	6,3 ^ Significant at the level of 1% probability (p <.01). ns — Not significant (p> = .05). by Scott-Knott test at 5% probability.
CV (%)		46,03	53,64

Table 5 Effect of substrates on the growth of seedlings of canafistula (Peltophorum dubium) (Spreng.) Taub, cutieira (Joannesia princeps Vell.) and rubber tree (Hevea brasiliensis M. Arg.) at the end of 180 days and of jatoba (Hymenaea courbaril L.) at the end of 210 days as to the production of arbuscular mycorrhizal fungi (AMF) spores and as to the production of arbuscular mycorrhizal fungi per weight of root dry matter (SPORES/RDM)

SUBSTRATES	SPECIES
SUBSTRATES	Canafistula	Cutieira	Jatobá	Rubber tree
SPORES
T1 - So	129,25 b	118 b	101 c	118,25 b
T2 - So+CF	97,25 b	94,5 b	81,66 c	131,5 b
T3: 80%So:20%Sa	67,75 b	459 b	89,75 c	177,25 b
T4: 80% So:20%Sa+CF	49,75 b	70 b	47 c	99,25 b
T5: 60%So:20%Sa: 20%PM	107,75 b	939 a	607 a	3748,25 a
T6: 50%So:20%Sa:30%PM	537,5 a	1064,25 a	265 b	821,5 b
T7: 40%So:20%Sa:40%PM	404,74 a	893,75 a	160,25 c	315,25 b
T8: 60%So:20%Sa:20%BM	103 b	129,75 b	72,25 c	124,5 b
T9: 50%So:20%Sa:30%BM	94 b	85,25 b	102 c	108,25 b
T10: 40%So:20%Sa:40%BM	51,75 b	171,75 b	173,75 c	227,75 b
CV%	44,63	51,93	23,23	45,93
SPORES/RDM
T1 - So	657,22 a	12,91 b	53,94 c	17,92 c
T2 - So+CF	266,78 a	8,97 b	56,6 c	17,32 c
T3: 80%So:20%Sa	111,33 a	39,2 a	45,45 c	21,52 c
T4: 80% So:20%Sa+CF	317,36 a	6,11 b	32,8 c	13,84 c
T5: 60%So:20%Sa: 20%PM	230,68 a	44,74 a	599,73 a	560,99 a
T6: 50%So:20%Sa:30%PM	332,55 a	86,17 a	300,52 b	167,1 b
T7: 40%So:20%Sa:40%PM	161,42 a	53,62 a	228,27 c	52,6 c
T8: 60%So:20%Sa:20%BM	149,43 a	10,2 b	42,85 c	15,54 c
T9: 50%So:20%Sa:30%BM	49,71 a	5,71 b	62,25 c	15,02 c
T10: 40%So:20%Sa:40%BM	32,58 a	13,5 b	225,56 b	28,14 c
CV%	55,19	48,78	41,87	51,86

[1] *Corresponding author.

Brasil

Brasil

EVALUATION OF SUBSTRATES AND AMF SPORULATION IN THE PRODUCTION OF SEEDLINGS OF NATIVE FOREST SPECIES

AVALIAÇÃO DE SUBSTRATOS E ESPORULAÇÃO DE FMAS NA PRODUÇÃO DE MUDAS DE ESPÉCIES FLORESTAIS NATIVAS

ABSTRACT