Revista do Colégio Brasileiro de Cirurgiões
versión On-line ISSN 1809-4546
MANTOVANI, Mario et al. Total hepatic warm ischemia and reperfusion associated with controlled hemorrhagic shock: effects of neutrophil sequestration in rat liver. Rev. Col. Bras. Cir. [online]. 2003, vol.30, n.4, pp. 275-281. ISSN 1809-4546. http://dx.doi.org/10.1590/S0100-69912003000400005.
BACKGROUND:The purpose of this experimental study was to evaluate the effects of total hepatic ischemia and reperfusion on the accumulation of neutrophils in the liver of rats, under normal conditions and in rats submitted to controlled hemorrhagic shock . METHODS: Thirty two adult male Wistar rats, were divided into four groups: the Control group, was submitted to the standard procedures for a period of 60 min of observation; Shock group, was submitted to controlled hemorrhagic shock (mean arterial blood pressure = 40 mmHg, 20 min) followed by volume resuscitation (lactated Ringer's solution + blood, 3:1) and reperfusion for 60 min; Pringle group, was submitted to total hepatic ischemia for 15 min and reperfusion for 60 min; The Total group, was submitted to controlled hemorrhagic shock for 15 min followed by volume resuscitation (lactated Ringer's solution + blood, 3:1), total hepatic ischemia for 15 min and reperfusion for 60 min. Measurements of serum lactate and base excess were used to characterize the hemorrhagic shock state with low tissue perfusion. The counting of neutrophils on the liver tissue was performed after the euthanasia of animals. RESULTS: Values for the counting of neutrophils on the liver indicate that, the animals from Pringle group differed from Shock and Total groups (Control 10.30±3.20, Shock 13.94±2.84, Pringle 7.00±3.40, Total 12.45±3.65) but did not differ from Control group. CONCLUSIONS: Rats submitted to controlled hemorrhagic shock state associated to total hepatic ischemia for 15 minutes, followed by 60 minutes of reperfusion, did not present significant neutrophils accumulation on liver tissue.
Palabras clave : Ischemia; Reperfusion; Shock, hemorrhagic; Liver; Neutrophil; Rats, wistar; Hypovolemia.