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Revista do Colégio Brasileiro de Cirurgiões

Print version ISSN 0100-6991On-line version ISSN 1809-4546


TODERKE, Edimar Leandro; BARETTA, Giorgio Alfredo Pedroso; GAMA FILHO, Ozimo Pereira  and  MATIAS, Jorge Eduardo Fouto. Sirolimus influence on hepatectomy-induced liver regeneration in rats. Rev. Col. Bras. Cir. [online]. 2014, vol.41, n.3, pp.203-207. ISSN 1809-4546.


To evaluate the influence of sirolimus on liver regeneration triggered by resection of 70% of the liver of adult rats.


we used 40 Wistar rats randomly divided into two groups (study and control), each group was divided into two equal subgroups according to the day of death (24 hours and seven days). Sirolimus was administered at a dose of 1mg/kg in the study group and the control group was given 1 ml of saline. The solutions were administered daily since three days before hepatectomy till the rats death to removal of the regenerated liver, conducted in 24 hours or 7 days after hepatectomy. Liver regeneration was measured by the KWON formula, by thenumber of mitotic figures (hematoxylin-eosin staining) and by the immunohistochemical markers PCNA and Ki-67.


there was a statistically significant difference between the 24h and the 7d groups. When comparing the study and control groups in the same period, there was a statistically significant variation only for Ki-67, in which there were increased numbers of hepatocytes in cell multiplication in the 7d study group compared with the 7d control group (p = 0.04).


there was no negative influence of sirolimus in liver regeneration and there was a positive partial effect at immunohistochemistry with Ki-67.

Keywords : Liver regeneration; Sirolimus; Hepatectomy; Proliferating cell nuclear antigen.

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