Analysis of natural history of the diaphragmatic injury on the right in mice

the animals were submitted to a surgical removal of portion from right hemi diaphragm by median laparotomy. The sample consists of 42 animals being 2 animals from pilot project and 40 operated animals. And the variables of the study were herniation, liver protection, healing, persistent diaphragm injury, evaluation of 16 channels tomography and the variables "heart rate" and "weight".

Results:

we analyzed 40 mice, we had two post-operative deaths; we had 17 animals in this group suffered from herniation (42.5%) and 23 animals didn't suffer from herniation (57.5%). Analyzing the tomography as image method in the evaluation of diaphragmatic hernia, we had as a method with good sensitivity (78.6%), good specificity (90.9%), and good accuracy (86.1%) when compared to necropsy.

Conclusion:

there was a predominance of healing of right hemi diaphragm, the size of initial injury didn't have influence on occurrence of the liver protection or hernia in mice.

Thoracic Surgery; Wound Healing; Diaphragm; Animal Experimentation; Animal Experimentation; Hernia, Diaphragmatic, Traumatic; Thoracic Injuries

Variable	Category	n	%

Macroscopy	Protection	23	57,5
Macroscopy	Hernia	17	42,5

Microscopy *	Protection	15	40,5
Microscopy *	Hernia	22	59,5

Hernia diagnosed by tomography **	Protection	23	63,9
Hernia diagnosed by tomography **	Hernia	13	36,1

Total		40	100

Group	n	Average	Standard deviation	Minimum	1^st Quartile	Median	3^rd Quartile	Maximum	p

Protection	23	10.48	3.72	5.76	6.03	9.41	12.75	19.37	0.530

Hernia	17	9.69	4.16	5.18	5.18	8.29	11.79	20.00	0.530

	Necropsy
	Protection	Hernia
	N	%	n	%

Microscopy -	Protection	15	75.0%	0	0.0%
Microscopy -	Hernia	5	25.0%	17	100.0%

Tomography -	Protection	20	90.9%	3	21.4%
Tomography -	Hernia	2	9.1%	11	78.6%

Assessment	Sensitivity	Specificity	Accuracy

Microscopy 95% CI	100.0%	75.0%	86.5%
Microscopy 95% CI	[81.57% ; 100.0%]	[53.1% ; 88.8%]	[72.0% ; 94.1%]
Tomography
Tomography	78.6%	90.9%	86.10%
95% CI	[52.4% ; 92.4%]	[72.2% ; 97.5%]	[71.3% ; 93.9%]

Variable/ Group	n	Average	Standard deviation	Minimum	1^st Quartile	Median	3^rd Quartile	Maximum

Size of the initial diaphragmatic injury
Protection	15	10.11	3.73	5.76					6.76	9.41	12.62	19.37	0.866
Hernia	22	9.89	3.95	5.18	6.76	8.50	11.79	20.00					0.866

Membrane thickness in mm (Microscopy)
Protection									15	0.25	0.32	0.01	0.08	0.11	0.20	1.00	0.379
Hernia	22	0.16	0.32	0.00	0.03	0.05	0.10	1.50									0.379

Fibrosis size in mm (Microscopy)
Protection									15	0.61	0.41	0.15	0.30	0.45	0.90	1.40	0.645
Hernia	22	0.70	0.68	0.20	0.30	0.48	0.82	3.30									0.645

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