Effects of heparin and hyperbaric oxygenation on necrosis reduction in an animal model for degloving injuries

ABSTRACT

Objective:

to evaluate the efficacy of the treatment with hyperbaric oxygen therapy or with topical and intralesional heparin in an animal model of degloving lesions.

Methods:

we conducted an experimental study with adult, male Wistar rats submitted to degloving of the left hind limb and divided into four groups according to the treatment: Group 1 (control) - without treatment; Group 2 (Heparin) - intralesional application at the time of surgery and topically, in the postoperative period, with heparin spray 10,000IU/mL; Group 3 (hyperbaric oxygenation) - daily sessions of 30 minutes in a hyperbaric chamber with 100% oxygen and 2 ATA pressure; Group 4 (positive control) - administration of a single dose of 45 mg/kg of intraperitoneal allopurinol. On the seventh day, we killed the animals, removed the cutaneous flaps and measured the total and necrotic areas, as well as computed the percentage of necrotic area.

Results:

the mean percentage of necrosis in the control group was 56.03%; in the positive control group it was 51.36% (p<0.45); in the heparin group, 42.10% (p<0.07); and in the hyperbaric oxygen therapy group, 31.58% (p<0.01) .

Conclusion:

both hyperbaric oxygen and heparin therapies were effective in reducing the percentage of necrosis in the model studied, although only the hyperbaric oxygenation showed statistical significance.

Keywords:
Surgery; Plastic. Wounds and Injuries. Traumatology