Pesquisa Veterinária Brasileira
versão impressa ISSN 0100-736X
CORTEZ, Amanda C. et al. Histomorfometric study of the spleen of healthy and diabetic Wistar rats supplemented or not with vitamin C. Pesq. Vet. Bras. [online]. 2009, vol.29, n.10, pp. 834-840. ISSN 0100-736X. http://dx.doi.org/10.1590/S0100-736X2009001000010.
The objective of this study was to analyze morphometric histological shatters of the spleen of normal and diabetic animals, comparing the joined results and relating them in the sex and the suplementation of vitamin C. Had been used 32 Wistar rats, which had been analyzed: number of vases; the number of germinative folicules (white pulp); and the diameter of the vases of each animal. Histological and morphometric analyses were applied on 5µm thick samples and showed that: in the amount of germinative folicules, we observe resulted comparing, independent of the sex, animals controls supplemented with vitamin C and controls not supplemented (p£0.05; F=0.1452); in the amount of vases, we observe resulted comparing, of diabetic females supplemented by vitamin C when compared with not supplemented (pd"0.05; F=6.8893); and in the diameter of the vases, we observe resulted comparing females, as much in the group has controlled how much to the diabetic group, supplemented with vitamin C when compared with the females not supplemented with vitamin C (p<0.05; U=121.50; Z(U)=2.1234) and (p<0,05; F=4.8134). In a general way, the induction of diabetes modifies the vascular diameter in the females and that the vitamin C administration only intervenes with relative metric data to the vascular diameter in the females.
Palavras-chave : Wistar rats; Diabetes mellitus; Spleen; Vitamin C.