SciELO - Scientific Electronic Library Online

 
vol.31 issue7Serum protein concentrations, including acute phase proteins, in calves experimentally infected with Salmonella DublinSearch agglutinins, leptospires antigen and apoptosis in the kidney of swine naturally infected with Leptospira spp. author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Pesquisa Veterinária Brasileira

Print version ISSN 0100-736X

Abstract

RODRIGUES, Rogério O. et al. Characterization of outer membrane proteins of serovar Hardjo isolated from cattle in Minas Gerais, Brazil. Pesq. Vet. Bras. [online]. 2011, vol.31, n.7, pp.555-560. ISSN 0100-736X.  https://doi.org/10.1590/S0100-736X2011000700002.

Differences in the protein profile of Leptospira sp. strains Sponselee, Norma and Hardjoprajitno were observed, with bands ranging from 175.47 kDa to 12.10 kDa. Strain Sponselee presented a 12-band profile, while strain Norma showed 11 and strain Hardjoprajitno showed 9 bands in the profile. All bands observed in Sponselee strain profile could match bands in the other two strains. Strain Norma lacks a band at 35.77 kDa and strain Hardjoprajitno lacks the bands at 89.59 kDa, 35.77 kDa and 12.10 kDa. The recognition profile from hyperimmune sera was also different for the studied serovar Hadjo strains. The majority of recognized proteins was in the range of 35.83 kDa to 29.19 kDa. Cattle sera against strain Norma only recognized low molecular mass proteins in strains Norma (6.80 kDa) and Hardjoprajitno (6.80 kDa and 5.30 kDa). Bovine sera against strain Hardjoprajitno recognized a 44.33 kDa protein in all studied strains and proteins of 4.22 kDa in strains Sponselee and Norma and of 10.49 kDa and 6.16 kDa in strain Hadjoprajitno. The different identified proteins could become specific targets to the development of diagnostic tests and vaccines against bovine leptospirosis.

Keywords : Leptospira serovar Hardjo; Minas Gerais State; Brazil; protein profile; cattle.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License