Pesquisa Veterinária Brasileira
Print version ISSN 0100-736X
CHIARELI, Denise et al. Control of leptospirosis in dairy cattle with autogenous vaccine in Santo Antônio do Monte, MG, Brazil. Pesq. Vet. Bras. [online]. 2012, vol.32, n.7, pp. 633-639. ISSN 0100-736X. http://dx.doi.org/10.1590/S0100-736X2012000700008.
An outbreak of leptospirosis in dairy cattle was observed in Santo Antonio do Monte, Minas Gerais. The herd had positive reactions in anti-Leptospira serovar Hardjo agglutination test (MAT) and had been previously vaccinated with a vaccine containing serovars Hardjo. The MAT showed 48.06% of cattle positive for serovars Hardjo genotype Hardjobovis, 36.82% for serovars Hardjo genotype Hardjoprajitno. The animals had abortions and mastitis with blood in the milk. This study aimed to isolate the existing serovars from the urine of serologically positive cows, produce an experimental vaccine with the serovars isolated in the herd, evaluating the effectiveness of the vaccination program for a period of two years through the herd serology. Leptospira spp. was isolated from the urine of two cows with signs suggestive of the disease. The strains were identified by serology with monoclonal antibodies and 16S rRNA gene sequencing as belonging to the Leptospira interrogans species Sejroe serogroup Hardjo serovars and Hardjoprajitno genotype. Use of the autochthonous vaccine was effective in leptospirosis controlling in the herd in two years. The serology results showed the absence of positive animals in the last race held in the herd.
Keywords : Leptospira; Hardjo; leptospirosis; cattle; autogenous vaccine; isolation.