SciELO - Scientific Electronic Library Online

 
vol.36 número2Utilização de plasma rico em plaquetas para estimulação da angiogênese em flape de padrão axial toracodorsal em coelhos (Oryctolagus cuniculus)Descrição anatômica de esqueletos de papagaios do gênero Amazona através da utilização de radiografias índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Pesquisa Veterinária Brasileira

versão impressa ISSN 0100-736Xversão On-line ISSN 1678-5150

Resumo

BRANCO, Érika et al. Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops. Pesq. Vet. Bras. [online]. 2016, vol.36, n.2, pp.119-122. ISSN 0100-736X.  http://dx.doi.org/10.1590/S0100-736X2016000200009.

Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.

Palavras-chave : Bone marrow; stem cell; mononuclear cells; Chlorocebus aethiops.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf )