SciELO - Scientific Electronic Library Online

 
vol.37 issue7Experimental poisoning by Niedenzuella stannea (Malpighiaceae) in sheepFrequency of toxin-encoding genes in Staphylococcus aureus isolated from community milk tanks author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Pesquisa Veterinária Brasileira

Print version ISSN 0100-736XOn-line version ISSN 1678-5150

Abstract

SANTOS, Fabrine A. et al. Microbiological, molecular and histopathological findings in small ruminants experimentally infected with Actinobacillus seminis. Pesq. Vet. Bras. [online]. 2017, vol.37, n.7, pp.686-690. ISSN 0100-736X.  https://doi.org/10.1590/s0100-736x2017000700006.

The aim of this study was to evaluate, in sheep, the pathogenicity of an Actinobacillus seminis strain isolated from a goat in Brazil. Samples of semen, puncture and fragments of epididymis, deferent duct, testicles and seminal vesicles from two goats (animals 1 and 2) and two sheep (animals 3 and 4) were used, and histopathological, microbiological culture and molecular diagnoses were performed. The inoculum was prepared with saline solution at 10-2 dilution corresponding to 1.0 McFarland standard, with A. seminis colonies previously cultured and administered on 2mL volume by intra-preputial (animals 1 and 3) and epididymis tail (animals 2 and 4) routes. At clinical evaluation it were found unilateral swelling of firm consistency after 30 days in epididymis and testicle from animal 4 that continued until the day of euthanasia, as well as animal 1 shown discrete unilateral swelling of testicles. Gross and microscopic lesions in animals 3 and 4 were compatibles with that caused by A. seminis infection. A. seminis was isolated from material of puncture and semen of one sheep (animal 4). It is concluded that the experimental infection model using goats and sheep has proved the pathogenicity of the A. seminis strain isolated from a goat in the Brazilian semiarid and reproduced in a sheep, which confirm the prediletion of the agent for epididymis, with clinical signs, histopathological findings, bacterial isolation and positive molecular diagnosis.

Keywords : Epididymitis; Actinobacillus seminis; experimental infection.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )