SciELO - Scientific Electronic Library Online

 
vol.21 issue2Teor de flavonóides e fenóis totais em folhas de Pyrostegia venusta Miers. de mata e de cerradoReserve substances and storage of Cyathea delgadii Sternb. spores author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Botany

On-line version ISSN 1806-9959

Abstract

MARQUES, DANIELA DE ARGOLLO; SHEPHERD, SIMONE LILIANE KIRSZENZAFT  and  CROCOMO, OTTO JESU. Influência de fontes de nitrogênio sobre o desenvolvimento das gemas axilares de explantes caulinares de Chrysanthemum morifolium Ramat. cultivado in vitro. Rev. bras. Bot. [online]. 1998, vol.21, n.2, pp. 141-147. ISSN 1806-9959.  http://dx.doi.org/10.1590/S0100-84041998000200005.

The effect of different nitrogen sources on the induction and rooting of axillary shoots in Chrysanthemum morifolium was tested. The total nitrogen concentration was maintained constant (60 mM) in all treatments, only the nitrogen source varying. Treatment E1 was a complete MS (Murashige & Skoog 1962) medium. The remaining treatments were: E2 = ammonium; E3 = nitrate; E4 = nitrate+urea (1.65 mM); E5 = nitrate+urea (3.33 mM); E6 = urea; E7 = urea+ glutamine, and E8 = glutamine. No phytohormones were added to the media. In treatment E1, both shoot and root development were promoted. The use of nitrate as the only source of nitrogen (treatment E3) was sufficient to maintain vigorous growth and no significant difference was found in any of the parameters measured when compared with the basic MS medium. The presence of urea promoted root development in Chrysanthemum, especially when used in addition to nitrate (treatments E4 and E5), hereby producing a dense root system. Both glutamine (treatment E7) and ammonium (treatment E2) were inefficient when used as the sole nitrogen source. Both of these sources resulted in a less vigorous plant growth, this being reflected in lower values for all of the parameters measured.

Keywords : Chrysanthemum; nitrogen sources; multiplication in vitro.

        · abstract in Portuguese     · text in Portuguese