Brazilian Journal of Botany
On-line version ISSN 1806-9959
OLIVEIRA JUNIOR, Clovis José Fernandes de; BRAGA, Marcia Regina and BUCKERIDGE, Marcos Silveira. Isolation of xyloglucan oligossacharides from dicots using enzymatic hydrolyses and gel permeation chromatography. Rev. bras. Bot. [online]. 2006, vol.29, n.3, pp. 391-397. ISSN 1806-9959. http://dx.doi.org/10.1590/S0100-84042006000300006.
Oligosaccharides from xyloglucan, a plant cell wall polysaccharide, have been considered as signaling molecules capable of regulating cell growth and expansion. The purification of such oligosaccharides is an essential requirement to evaluate their biological activity. In the present work, xyloglucans extracted from seeds of copaiba (Copaifera langsdorffii Desf.) and bean cell suspension cultures (Phaseolus vulgaris L.) were hydrolyzed with cellulase (endo-glucanase) to obtain xyloglucan oligosaccharides (OXGs). The OXGs were separated by gel permeation chromatography and analysed by high performance anion exchange chromatography with pulse amperometric detector (HPAEC/PAD). The results indicate that these procedures were suitable for purification of OXGs since XLLG and XXXG were 90% and XXFG 80% pure, showing the expected biological activity.
Keywords : cell wall; oligosaccharides; purification; xyloglucan.