SciELO - Scientific Electronic Library Online

 
vol.29 issue3Pollination by hummingbirds in a "caatinga" area in Pernambuco State, northeastern BrazilPhytoplankton in three lateral lakes and in the Paranapanema River in its mouth zone into Jurumirim Reservoir, São Paulo, Brazil, during a long drought period author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Botany

Print version ISSN 0100-8404On-line version ISSN 1806-9959

Abstract

OLIVEIRA JUNIOR, Clovis José Fernandes de; BRAGA, Marcia Regina  and  BUCKERIDGE, Marcos Silveira. Isolation of xyloglucan oligossacharides from dicots using enzymatic hydrolyses and gel permeation chromatography. Rev. bras. Bot. [online]. 2006, vol.29, n.3, pp.391-397. ISSN 0100-8404.  http://dx.doi.org/10.1590/S0100-84042006000300006.

Oligosaccharides from xyloglucan, a plant cell wall polysaccharide, have been considered as signaling molecules capable of regulating cell growth and expansion. The purification of such oligosaccharides is an essential requirement to evaluate their biological activity. In the present work, xyloglucans extracted from seeds of copaiba (Copaifera langsdorffii Desf.) and bean cell suspension cultures (Phaseolus vulgaris L.) were hydrolyzed with cellulase (endo-glucanase) to obtain xyloglucan oligosaccharides (OXGs). The OXGs were separated by gel permeation chromatography and analysed by high performance anion exchange chromatography with pulse amperometric detector (HPAEC/PAD). The results indicate that these procedures were suitable for purification of OXGs since XLLG and XXXG were 90% and XXFG 80% pure, showing the expected biological activity.

Keywords : cell wall; oligosaccharides; purification; xyloglucan.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License