Brazilian Journal of Genetics
On-line version ISSN 1678-4502
WALDSCHMIDT, Ana Maria; SALOMAO, Tânia Maria Fernandes; BARROS, Everaldo Gonçalves de and CAMPOS, Lúcio de Antônio Oliveira. Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae). Braz. J. Genet. [online]. 1997, vol.20, n.3, pp.-. ISSN 1678-4502. http://dx.doi.org/10.1590/S0100-84551997000300011.
The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvae