Brazilian Journal of Genetics
On-line version ISSN 1678-4502
VALARINI, Maria José; OTSUK, Ivani Pozar and VIEIRA, Maria Lucia Carneiro. Changes in N2 fixation in Stylosanthes scabra derived from tissue culture. Braz. J. Genet. [online]. 1997, vol.20, n.4 ISSN 1678-4502. http://dx.doi.org/10.1590/S0100-84551997000400025.
Plants were regenerated from leaf-derived callus culture of Stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. A total of 168 regenerants were planted out in Leonard jars in a complete randomized design. Nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot N content and acetylene reduction activity. The results showed higher variation in the regenerants than in controls not submitted to tissue culture. Significant differences were found for all nitrogen fixation related-traits