Brazilian Journal of Medical and Biological Research
On-line version ISSN 1414-431X
MORAES, M.T.B.; NIEL, C. and GOMES, S.A.. A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus. Braz J Med Biol Res [online]. 1999, vol.32, n.1, pp.45-49. ISSN 1414-431X. http://dx.doi.org/10.1590/S0100-879X1999000100006.
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.
Keywords : hepatitis B virus; genotype F; subtype adw4; polymerase chain reaction; Brazil.