Brazilian Journal of Medical and Biological Research
On-line version ISSN 1414-431X
SCHRODER, N. et al. Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats. Braz J Med Biol Res [online]. 2000, vol.33, n.2, pp.229-232. ISSN 1414-431X. http://dx.doi.org/10.1590/S0100-879X2000000200011.
Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P<0.01), but not at 0, 15, or 60 min after training, and did not affect glutamate binding in membranes obtained from the parietal cortex. These results are consistent with previous evidence for the involvement of glutamatergic synaptic modification in the hippocampus in the early steps of memory formation.
Keywords : glutamate receptor; hippocampus; parietal cortex; long-term potentiation; emotional learning, memory.