Brazilian Journal of Medical and Biological Research
versão On-line ISSN 1414-431X
CAMARGO, A.A. et al. Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12. Braz J Med Biol Res [online]. 2001, vol.34, n.10, pp. 1237-1245. ISSN 1414-431X. http://dx.doi.org/10.1590/S0100-879X2001001000002.
DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.
Palavras-chave : DDX26; DEAD-box RNA helicase; 7p12; ribosome biogenesis.