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Brazilian Journal of Medical and Biological Research

On-line version ISSN 1414-431X

Abstract

CUI, Xin et al. Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein. Braz J Med Biol Res [online]. 2011, vol.44, n.12, pp. 1251-1255.  Epub Oct 28, 2011 ISSN 1414-431X.  http://dx.doi.org/10.1590/S0100-879X2011007500144.

Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b’) present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b’ genes may determine the manifestations of HCMV infection in vivo. However, little is known about the host factors, which interact with UL/b’ proteins. This study was conducted to investigate the function of the HCMV UL136 protein. By yeast two-hybrid screening, the β1 subunit of the host Na+/K+-ATPase (ATP1B1) was identified to be a candidate protein, which interacts with the HCMV UL136 protein. The interaction was further evaluated both in vitro by pull-down assay and in vivo by immunofluorescent co-localization. The results showed that the UL136 protein can interact with ATP1B1 in vitro. Co-localization of UL136-EGFP and ATP1B1-DsRed in cell membranes suggests that ATP1B1 was a partner of the UL136 protein. It can be proposed that the HCMV UL136 protein may have important roles in processes such as cell-to-cell spread, and in maintaining cell osmotic pressure and intracellular ion homeostasis during HCMV infection.

Keywords : HCMV; UL136; ATPase; Yeast two hybridization.

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