SciELO - Scientific Electronic Library Online

 
vol.20 issue3Occurrence of Bacillus sporothermodurans and the influence of the thermal processing procedure on its presence in brazilian UHT milkExtraneous materials in ground cinnamon and ground paprika commercialized in São Paulo state author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Food Science and Technology

Print version ISSN 0101-2061On-line version ISSN 1678-457X

Abstract

OLIVEIRA, Marize Silva; PRADO, Guilherme  and  JUNQUEIRA, Roberto Gonçalves. Comparison of thin layer chromatography and ELISA techniques in the quantification of aflatoxins in corn samples. Ciênc. Tecnol. Aliment. [online]. 2000, vol.20, n.3, pp.369-374. ISSN 0101-2061.  https://doi.org/10.1590/S0101-20612000000300015.

Two methods, enzyme linked immunosorbent assay (ELISA) and thin layer chromatography (TLC) by visual and densitometric, were compared for determination of aflatoxins in corn samples. The highest incidence of total aflatoxin detected by those methods were ranged from 0-30 mg/kg and above 300 mg/kg. For aflatoxins B2, G1, G2 the levels were ranged from 0-30mg/kg. The samples results showed variation coefficient below of 20, 30 and 40% for ELISA and CCD with densitometric and visual quantification, respectively. The correlation coefficients were highly significative for the relation between visual quantification and densitometric (r = 0,9219; t = 28,36; p<0,001), ELISA and visual quantification (r =0,8277; t = 17,58; p< 0,001), ELISA e quantification densitometric (r = 0,7373; t=13,01; p< 0,001), in the total aflatoxin detemination of all corn samples. confirming the equivalence of the studied methods.

Keywords : Aflatoxin; Thin layer chromatography; ELISA; corn.

        · abstract in Portuguese     · text in Portuguese

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License