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vol.21 issue2DEHYDRATION OF ACEROLA PULP (Malpighia emarginata D.C.) BY FOAM-MAT DRYING PROCESSSTUDIES ON THE USE OF JATOBA FLOUR IN BISCUITS AS A SOURCE OF DIETARY FIBRE CONTAINING NO ADDED SIMPLE SUGARS author indexsubject indexarticles search
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Food Science and Technology

Print version ISSN 0101-2061On-line version ISSN 1678-457X

Abstract

PEREIRA, Maria Lúcia; CARMO, Luiz Simeão  and  PEREIRA, José Luiz. EVALUATION OF GROWTH AND ENTEROTOXIGENIC CAPACITY OF COAGULASE-NEGATIVE LOW-ENTEROTOXIN-PRODUCING STAPHYLOCOCCAL STRAINS IN EXPERIMENTALLY INOCULATED FOOD. Ciênc. Tecnol. Aliment. [online]. 2001, vol.21, n.2, pp.171-175. ISSN 0101-2061.  http://dx.doi.org/10.1590/S0101-20612001000200009.

Fourteen coagulase-negative low-enterotoxin-producing staphylococcal strains ¾ S.caprae; S.chromogenes; S.cohnii; S.epidermidis; S.haemolyticus, two strains; S.hyicus; S.lentus; S.sciuri; S.xylosus, two strains; e S.warneri, two strains ¾ with capacity of synthesis ranging from 1.4 to 16.8ng of staphylococal enterotoxin/mL were obtained from anatomic sites of healthy goats in Spain. Those staphylococcal strains were used with the aim of evaluating their growth and enterotoxigenic capacity in experimentally inoculated foods. Samples of whole milk and cooked ham were inoculated with 104UFC Staphylococci/g ormL. These substrates were kept at 30°C, during 24 and 48 hours and submitted to the enumeration of staphylococcal cells on Baird Parker agar. The enterotoxin detection was made by ELISA and RPLA. The results revealed, concerning to staphylococcal behaviour in whole milk and cooked ham, that all strains showed development range of 106 to 109CFU/g ormL after 48 hours incubation and no SE production was observed in the inolculated substrates that could be detected by ELISA, SET-EIA and RPLA.

Keywords : coagulase-negative low-staphylococcal; enterotoxins.

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