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Food Science and Technology

Print version ISSN 0101-2061On-line version ISSN 1678-457X

Abstract

MERCADANTE, Adriana Z.  and  RODRIGUEZ-AMAYA, Délia. CONFIRMATION OF THE IDENTITY OF a-CRYPTOXANTHIN AND INCIDENCE OF MINOR PROVITAMIN A CAROTENOIDS IN GREEN LEAFY VEGETABLES. Ciênc. Tecnol. Aliment. [online]. 2001, vol.21, n.2, pp.216-222. ISSN 0101-2061.  http://dx.doi.org/10.1590/S0101-20612001000200017.

The main carotenoids from green leafy vegetables have been consistently found to be lutein, b-carotene, violaxanthin and neoxanthin. However, there is a controversy about the identity of minor carotenoids. Therefore, electron impact mass spectrometry and high performance liquid chromatography with a diode array detector were used in order to confirm the identity of the minor provitamin A carotenoids in Brazilian green leaves. The provitamin A carotenoids, including the cis and trans isomers of b-carotene, were separated on a polymeric C18 column, Vydac 201TP54, with MeOH/H2O (98:2) as mobile phase. The UV-visible and mass spectra confirmed that the monohydroxy carotenoid present in Brazilian green leafy vegetable to be a-cryptoxanthin, and not b-cryptoxanthin as reported in the international literature. All eleven green leaves analyzed (water-cress, unheaded lettuce, lettuce, wild chicory, "caruru", common chicory, kale, spinach, endive, roquette, parsley, and "taioba") had a-cryptoxanthin, 9-cis and 13-cis- b-carotene, whereas a-carotene was found in only four of these leaves ("caruru", kale, salsinha and "taioba").

Keywords : carotenoids; mass spectrometry; high performance liquid chromatography; green leafy vegetables.

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