Food Science and Technology (Campinas)
On-line version ISSN 1678-457X
NEVES, Valdir A.; SILVA, Maraiza A. da and LOURENCO, Euclides J.. Characterization and in vitro tryptic hydrolysis of the major globulin from chickpea (Cicer arietinum L.). Ciênc. Tecnol. Aliment. [online]. 2004, vol.24, n.1, pp. 139-145. ISSN 1678-457X. http://dx.doi.org/10.1590/S0101-20612004000100025.
The isolation and characterization of the major globulin fraction (11 S) from Chickpea, vc IAC-Marrocos, were evaluated. The major globulin was extracted, isolated by gel filtration and ion-exchange chromatography showing only one protein band on PAGE. The globulin, after Sephadex elution, revealed two protein bands of 55 and 52.5kDa and three minor bands on SDS-PAGE. In the presence of 2-mercaptoethanol six polypeptides were revealed on SDS-PAGE in the range of 18 to 42kDa. The isolated native globulin shown to be resistant to trypsin and chymotrypsin however heating at 96 and 121ºC/15min was not sufficient to increase the hydrolysis significantly. The proteolytic susceptibility of the enzymes was reduced by 0.3M NaCl addition at the assay. The salt concentration was sufficient to stabilize the native protein structure that was lost after heating as demonstrated on SDS-PAGE.
Keywords : chickpea; Cicer arietinum L.; major globulin; characterization; in vitro hydrolysis.