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Food Science and Technology (Campinas)

Print version ISSN 0101-2061

Abstract

MARTINEZ, Ernesto Acosta  and  SANTOS, Juliana Aparecida Frade. Influence of the use of rice bran extract as a source of nutrients on xylitol production. Ciênc. Tecnol. Aliment. [online]. 2012, vol.32, n.2, pp. 308-313.  Epub Mar 20, 2012. ISSN 0101-2061.  http://dx.doi.org/10.1590/S0101-20612012005000036.

Xylose-to-xylitol bioconversion using 2.5 or 10% (v/v) rice bran extract was performed to verify the influence of this source of nutrients on Candida guilliermondii metabolism. Semisynthetic medium (SM) and sugarcane bagasse hemicellulosic hydrolysate detoxified with ion-exchange resins (HIE) or with alteration in pH combined with adsorption onto activated charcoal (HAC) were fermented in 125 mL Erlenmeyer flasks at 30 ºC and 200 rpm for 72 hours. Activated charcoal supplemented with 2.5% (v/v) rice bran extract was fermented by C. guilliermondii in a MULTIGEN stirred tank reactor using pH 5.0 and 22.9/hour oxygen transfer volumetric coefficient. Higher values of xylitol productivity (0.70, 0.71, and 0.62 g.Lh-1) and xylose-to-xylitol conversion yield (0.71, 0.69, and 0.63 g.g-1) were obtained with 2.5% (v/v) rice bran in semisynthetic medium, ion-exchange resins, and activated charcoal, respectively. Moreover, during batch fermentation, the xylitol volumetric productivity and fermentation efficiency values obtained were 0.53 g.Lh-1 and 61.1%, respectively.

Keywords : hemicellulosic hydrolysate; sugarcane bagasse; xylose; xylitol; rice bran.

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