Biomass production by <i>Arthrospira platensis</i> under different
          culture conditions

Castro, Gabriela Fernandes Pepe da Silva de; Rizzo, Roberta Ferreira; Passos, Thaís Souza; Santos, Beatriz Nascimento Corrêa dos; Dias, Daiana da Silva; Domingues, Josiane Roberto; Araújo, Kátia Gomes de Lima

doi:10.1590/1678-457X.6421

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Research Paper • Food Sci. Technol (Campinas) 35 (1) • Jan-Mar 2015 • https://doi.org/10.1590/1678-457X.6421 copy

Biomass production by Arthrospira platensis under different culture conditions

Authorship SCIMAGO INSTITUTIONS RANKINGS

In biotechnological processes, the culture media components are responsible for high costs and exert a strong influence on the cyanobacteria behavior. The objective of this study was to evaluate the Arthrospira platensis growth potential for biomass production under different cultivation conditions using an experimental design. Three factors that are important for cyanobacteria growth were evaluated: sodium bicarbonate (9 to 18 g/l), sodium nitrate (1.25 to 2.5 g/l), and irradiance (20 to 120 µmol photons/m².s^–1). The results showed that the concentration of NaNO₃ in the A. platensis medium can be reduced, resulting in increased concentrations of biomass produced. There was a higher biomass production due to the increase in the concentration of NaHCO₃ and irradiance, mainly when these two factors varied tending towards the highest values studied. The results demonstrate the potential to produce Arthrospira platensis with lower costs and effluent generation without affecting cultivation performance.

cyanobacteria; culture medium; sodium bicarbonate; sodium nitrate; irradiance

Parameters	–α	–1	0	1	₊α
Sodium bicarbonate (g/l)	9	10.82	13.5	16.18	18
Sodium nitrate (g/l)	1.25	1.5	1.875	2.25	2.5
Irradiance (µmol photons/m².s^–1)	20	28.33	70	111.67	120

Experiment (E)	Sodium bicarbonate (g/l)	Sodium nitrate (g/l)	Irradiance (µmol photons/m².s^–1)	Biomass production (mg/l)	Productivity (mg/l/day) P₂₁
1	10.82	1.500	28.33	1,070	48.571
2	16.18	1.500	28.33	1,540	70.952
3	10.82	2.250	28.33	1,060	48.095
4	16.18	2.250	28.33	1,000	45.238
5	10.82	1.500	111.67	2,380	110.952
6	16.18	1.500	111.67	3,270	153.333
7	10.82	2.250	111.67	2,210	102.857
8	16.18	2.250	111.67	2,150	100.000
9	9.00	1.875	70.00	2,050	95.238
10	18.00	1.875	70.00	1,625	75.000
11	13.50	1.250	70.00	1,980	91.905
12	13.50	2.500	70.00	1,910	88.571
13	13.50	1.875	20.00	1,390	63.809
14	13.50	1.875	120.00	2,240	104.286
15	13.50	1.875	70.00	1,790	82.857
16	13.50	1.875	70.00	1,930	89.524
17	13.50	1.875	70.00	2,050	95.238

Factors	Regression coefficients	Standard error	t(2)	p-value	Estimates interval (90%)
Factors	Regression coefficients	Standard error	t(2)	p-value	Lower limit	Upper limit
Average	1859.09	39.24	47.38	0.00	1744.52	1973.66
NaHCO₃	667.50	46.01	14.51	0.00	533.16	801.84
NaNO₃	–230.00	46.01	–5.00	0.04	–364.34	–95.66
Irradiance	155.00	46.01	3.37	0.08	20.66	289.34
NaNO₃ / Irradiance	–185.00	46.01	–4.02	0.06	–319.34	–50.66

Source of variation	Sum of squares	Degrees of freedom	Mean square	F calc	p-value
Regression	4334.09	4	1083.52	22.42	< 0.1
Residual	290.00	6	48.33
Total	4624.09	10	320.50

E	Sodium bicarbonate (g/l)	Sodium nitrate (g/l)	Irradiance (µmol photons/m².s^–1)	Total protein (%)	Phycocyanin produced (%)	Allophycocyanin produced (%)
4	16.18	2.25	28.33	60.84	8.27	4.20
6	16.18	1.5	111.67	46.19	7.35	2.95

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[1] *Corresponding author: gabriela_pepe@hotmail.com