Revista Brasileira de Sementes
Print version ISSN 0101-3122
BINNECK, Eliseu et al. Electrophoretic patterns of hordeins and isoenzymes for identification of barley cultivars. Rev. bras. sementes [online]. 2002, vol.24, n.1, pp. 312-321. ISSN 0101-3122. http://dx.doi.org/10.1590/S0101-31222002000100043.
The goal of this study was to determine the polyacrylamide gel electrophoretic patterns (electrophoregrams) of the hordeínas and isozymes from five Brazilian barley cultivars: Embrapa 43, Embrapa 127, Embrapa 128, Embrapa 129 e BR 2. The uniformity (homogeneity) of these patterns also was determined through the 90 single seed samples of each cultivar. Most of the cultivars were readily discriminated by hordein electrophoregrams; five barley cultivars presented four different eletroforegrams of hordeins. Three cultivars gave unique hordein patterns, whereas the two others (Embrapa 128 e BR 2) presented different esterases (EC 18.104.22.168) patterns. Jaccard similarity measures of conjugated data of hordeínas and esterases allowed discrimination of all cultivars and biotypes. The cultivar Embrapa 43 showed two biotypes well defined of hordeins and esterases. The variation of the hodeins pattern on that cultivar have direct relation with the variation on zimograms of esterases. This relation was not found in all cultivars that presented biotypes. It was established that electrophoresis data of hordeins and esterases can be helpful on barley cultivars identification.
Keywords : cultivar identification; PAGE; prolamins; esterase; Hordeum vulgare L..