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Revista Brasileira de Sementes

versión impresa ISSN 0101-3122


MENEZES, Sabrina Mecca de; TILLMANN, Maria Ângela Andre; DODE, Luciana Bicca  y  VILLELA, Francisco Amaral. Detection of soybean genetically modified tolerant to glyphosate methods based on the activity of enzymes. Rev. bras. sementes [online]. 2004, vol.26, n.2, pp.150-155. ISSN 0101-3122.

Progress in genetic engineering allow the production of genetically modified plants tolerant to the use of certain herbicides. The use of seeds of genetically modified plants is increasing quickly, and fast, practical and low cost detection methods need to be developed. The objective of this research was to establish a method for detection of soybean tolerant to glyphosate. Two soy cultivars were compared, one tolerant to glyphosate and its genetically unmodified parent. The seeds were submitted to treatments of pre-imbibition for 16 hours, in water and herbicide solution containing 0,6% of the active ingredient at the temperature of 25°C for seven days. Afterwards, the extraction and the determination of the activity of the enzyme peroxidase were carried out through the evaluation of the eletroforesis and of color reaction. The obtained results allowed the conclusion that it is possible to differentiate soy tolerance to herbicide glyphosate through color reaction. Differences were observed in the enzyme peroxidase activity tolerant and susceptible to glyphosate cultivars.

Palabras clave : Glycine max; transgenic; herbicide.

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