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Revista Brasileira de Sementes

versão impressa ISSN 0101-3122

Resumo

JOSE, Anderson C. et al. Changes in gene expression during drying and imbibition of desiccation sensitive Magnolia ovata (A. St.-Hil.) spreng. seeds. Rev. bras. sementes [online]. 2009, vol.31, n.1, pp.270-280. ISSN 0101-3122.  https://doi.org/10.1590/S0101-31222009000100030.

Seeds of Magnolia ovata were dried to different water contents to assess the viability and transcript abundance of genes related to seed development, cell cycle, cytoskeleton and desiccation tolerance.The expression of development, cell cycle and cytoskeleton relative genes (ABI3, CDC2-like and ACT2) alone could not explain the germination behaviour of M. ovata seeds in relation to drying damage. Irrespective of their initial water content, the seeds performed in the same way during the initial period of germination and the deleterious effects of desiccation only occurred in later stages. Expression of PKABA1, sHSP17.5 and 2-Cys-PRX did not show a relationship with desiccation. However, the expression patterns of PKABA1 and sHSP17.5 suggested the participation of these genes in protective mechanisms during the imbibition of M. ovata seeds.

Palavras-chave : desiccation tolerance; drying; gene expression; Magnolia ovata.

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