Revista Brasileira de Sementes
versión impresa ISSN 0101-3122
HENNING, Fernando Augusto; MERTZ, Liliane Marcia; ZIMMER, Paulo Dejalma y TEPLIZKY, Marcus Davi Ferreira. Physiological, sanitary quality and isoenzyme analysis of black oat seeds treated with different fungicides. Rev. bras. sementes [online]. 2009, vol.31, n.3, pp. 63-69. ISSN 0101-3122. http://dx.doi.org/10.1590/S0101-31222009000300007.
Black-oat is a rustic grass used as livestock fodder as well as winter green fertilization in crop rotation systems. Low sanitary quality of black oat seeds due to inadequate handling at harvest and in after-harvest phase is one of the limiting factors to production. The objective of this study was to evaluate the performance of a bioprotetor (Trichoderma spp.) and chemical fungicides (Carbendazin + Thiram and Carboxin + Thiram) in the physiological and sanitary quality of black oat seeds and in their enzymatic activities. After the treatment the physiological quality of the seeds was evaluated by germination and vigor tests (speed of emergence and first germination count). The sanitary analysis was carried out by the blotter test. Alcohol desidrogenase, acid fosfatase and esterase activity were evaluated by electrophoresis. Vigour and germination tests showed that seeds treated with chemical fungicides performed better. In the sanitary analysis, the presence of fungi Bipolaris sorokiniana and Fusarium graminearum was observed. The treatment with bioprotetor did not differ statistically from the control, showing higher fungi incidence when compared to the seeds treated with chemical fungicides. In relation to biochemical alterations, the treated seeds and the ones treated with bioprotetor presented an increase in the isoenzymatic activity in all the evaluated enzymes indicating degenerative transformations due to fungi action.
Palabras llave : Avena strigosa; electrophoresis; seed treatment; bioprotetor.