Revista Brasileira de Sementes
Print version ISSN 0101-3122
PEDROSO, Daniele Cardoso et al. Inoculation methods of Alternaria alternata and A. dauci in parsley seeds and their influence on the physiological quality. Rev. bras. sementes [online]. 2010, vol.32, n.3, pp. 79-85. ISSN 0101-3122. http://dx.doi.org/10.1590/S0101-31222010000300009.
The objective of the study was to evaluate the effect of the association of Alternaria alternata and A. dauci on the physiologic quality of parsley seeds inoculated by two methods: conidia suspension and water restriction. For the inoculation with suspension, a solution containing 105 conidia of A. alternata, A. dauci and both species (A. alternata + A. dauci), was prepared in which the seeds remained for 30 minutes. For inoculation by water restriction, a PDA medium was prepared, supplemented with 33.1 g/L of manitol, in which the A. alternata, A. dauci and A. alternata + A. dauci colonies grew. The seeds remain on the plates for 48 hours. After the inoculation, the quality of the seeds was evaluated by testing in the laboratory and greenhouse. The parsley seeds had their physiological quality damaged by association with A. alternata and A. dauci under laboratory conditions, especially in relation to germination, first count germination, abnormal seedlings, percentage of dead seeds and the cold test.
Keywords : Petroselinum crispum; Alternaria spp; conidia suspension; water restriction; vigor.