SciELO - Scientific Electronic Library Online

vol.34 issue1Chemical and biological treatments of castor bean seeds: effects on germination, emergence and associated microorganismsPlantability and moisture content of naked and pelleted seeds of supersweet (Sh2) corn during cold storage conditions author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Sementes

Print version ISSN 0101-3122


SILVA, Mirian de Sousa; BROMMONSCHENKEL, Sérgio Hermínio; FARIA, José Márcio Rocha  and  BORGES, Eduardo Euclydes de Lima e. Partial characterization of genes from the embryonic axis of Melanoxylon brauna Schott. (Leguminosae-Caesalpinioideae) seeds. Rev. bras. sementes [online]. 2012, vol.34, n.1, pp.29-38. ISSN 0101-3122.

The objective of this study was to partially characterize some genes involved in the desiccation tolerance of the embryonic axis of Melanoxylon brauna seeds subjected, or not, to oven fast-drying. Seeds were initially dried rapidly in an oven at 40 ºC, 50 ºC, 60 ºC, 70 ºC, and 80 °C, for 24, 48 and 72 h and then subjected to germination tests and moisture content determination. Degenerate primers were designed for 19 genes. The CDNA was used as a template for PCR amplifications using the degenerate primers, and the PCR products obtained were purified, cloned and sequenced. The seeds showed a gradual reduction in percent germination with increasing temperature and drying time. Nucleotide sequences of the cloned fragments related to genes CAT1, SPS1, Abi5, Transk and PM25 were obtained. The similarity analysis with the sequences deposited in databases revealed similarities with genes CAT1, SPS1, Transk and PM25 from other plant species. The nucleotide sequences obtained from the respective genes will be used for designing specific primers for gene expression analyses during seed germination in order to understand the causes for loss of physiological quality of Melanoxylon brauna seeds.

Keywords : water; stress; drying; sequencing.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License