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Methodology for bioassay entomopathogenic Bacillus against sand fly (Diptera, Psychodidae, Phlebotominae) larvae

The objective of this research is to present a method to bioassay entomopathogenic Bacillus species against sand fly (Diptera, Psychodidae, Phlebotominae) larvae. The vector Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae) and some Bacillus strains were used in laboratory to develop this method. Some characteristics of this method are commented and the importance to develop a standard method to test pathogenicity of Bacillus species against phlebotomine larvae is also discussed.

Phlebotominae; Bacillus; bioassay; method


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