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Revista Brasileira de Zoologia

Print version ISSN 0101-8175


SOUZA, Valéria Nogueira de; MARTINS, Maurício Laterça; MORAES, Flávio Ruas de  and  KRONKA, Sérgio do Nascimento. Methodology of experimental infection and the susceptibility degree of the tambacu hybrid and Leporinus mucrocephalus Garavello & Britski to four inocula of trophonts of Ichtyophthirius multifiliis Fouquet. Rev. Bras. Zool. [online]. 2001, vol.18, n.3, pp.803-811. ISSN 0101-8175.

Tropical fishes like Leporinus macrocephalus Garavello & Britski, 1988 ("piauçu") and the hybrid "tambacu" (Piaractus mesopotamicus Holmberg, 1887 "pacu" male x Colossoma macropomum Cuvier, 1818 "tambaqui" female) were experimentally infected with four inocula of trophonts of Ichthyophthirius multifiliis Fouquet, 1876 (Protozoa, Ciliophora) and the infection level was evaluated. Each one of twelve experimental units consisted of a tank with 200 liters of water and two specimens of each kind of fish. The following treatments were designed to each tank, in triplicate: 8, 16,32 and 64 trophonts. After 8 days of exposure the fishes were killed and the number of trophonts was counted at a stereoscopic microscope. This assay showed to be feasible at tropical conditions and was able to show that "tambacu" was more susceptiblc than "piauçu".

Keywords : Ichthyophthirius multifiliis; tropical fish; experimental infection; susceptibility.

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