Revista Brasileira de Coloproctologia
Print version ISSN 0101-9880
LEAL, Raquel Franco et al. Inflammatory activity of pelvic ileal pouches mucosa in asymptomatic patients. Rev bras. colo-proctol. [online]. 2008, vol.28, n.4, pp. 443-448. ISSN 0101-9880. http://dx.doi.org/10.1590/S0101-98802008000400006.
Pouchitis after total retocolectomy is the most common complication in ulcerative colitis (UC). The immunological mechanisms involved in the genesis of pouchitis are unclear. PURPOSE: To evaluate STAT-1 activation and IFN-ã expression in normal ileal pouch mucosa. METHODS: Eighteen asymptomatic patients submitted to total retocolectomy and J pouch, were evaluated, being nine with UC and nine with FAP. The activation of STAT-1 and expressions of the cytokine were determined by immunoblot of total protein extracts from pouch mucosal biopsies. RESULTS: STAT-1 activation was increased in UC, when compared to FAP and controls. Higher levels of IFN-γ expression were observed in UC when compared to control group, but was similar to FAP. CONCLUSIONS: These findings could explain a higher susceptibility to this inflammatory complication in UC when compared to FAP, which may be similar to UC disease.
Keywords : Ulcerative colitis; familial adenomatous polyposis; ileal pouch; pouchitis; cytokines.