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Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Print version ISSN 0102-0935On-line version ISSN 1678-4162

Abstract

MORI, E.; MORI, C.M.C.  and  FERNANDES, W.R.. Evaluation of alveolar macrophage function in healthy horses. Arq. Bras. Med. Vet. Zootec. [online]. 2001, vol.53, n.2, pp.1-7. ISSN 0102-0935.  http://dx.doi.org/10.1590/S0102-09352001000200007.

Due to the importance of alveolar macrophages (AM) in pulmonary defense mechanisms, studies were performed in order to evaluate the activity of these cells. Bronchoalveolar lavages (BAL) were obtained from five healthy horses, and cytology was performed on glass slides after cytocentrifugation of the samples. Slides were stained by Rosenfeld. All BAL samples were centrifuged and cell concentration was adjusted to 2´106 cells/ml, for the measurement of AM activity (spreading, phagocytosis and hydrogen peroxide release tests). Differential counting of the BAL cells demonstrated that macrophages were the predominant type of cell (59.0± 6.9%). Measurement of AM activity presented the following results: spreading rate, 25.1± 19.7%, phagocytosis rate, 89.4± 6.2% and hydrogen peroxide release, 1.6± 0.3nmoles/2´105 cells (without PMA- phorbol 12-myristate 13-acetate) and 1.8± 0.4nmoles/2´105 cells (with PMA). Results presented a pattern for the activity of AM in healthy horses enabling the demonstration of an activation rate even without known previous eliciting factors. These results indicate that the tests of macrophage activity measurement are adequate for evaluation of phagocytic activity of AMs.

Keywords : Horse; alveolar macrophage; phagocytosis; bronchoalveolar lavage.

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