Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
CANDIDO, A.L.; RESENDE, M.; BESSA, L.R.G. and LEITE, R.C.. Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein. Arq. Bras. Med. Vet. Zootec. [online]. 2003, vol.55, n.3, pp. 256-261. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352003000300002.
A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a.
Keywords : bovine herpesvirus-1.2a; glycoprotein D gene; DNA vaccine; ELISA.