Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
DOMINGUES, S.F.S. et al. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles. Arq. Bras. Med. Vet. Zootec. [online]. 2003, vol.55, n.3, pp. 301-308. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352003000300009.
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
Keywords : monkey; preantral follicle; isolation; ovary; Cebus apella.