Arquivo Brasileiro de Medicina Veterinária e Zootecnia
On-line version ISSN 1678-4162
MORTOLA, E. et al. Feline immunodeficiency virusinfection: a comparative study of different diagnostic techniques. Arq. Bras. Med. Vet. Zootec. [online]. 2004, vol.56, n.1, pp.13-18. ISSN 1678-4162. http://dx.doi.org/10.1590/S0102-09352004000100003.
This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
Keywords : feline immunodeficiency virus; immunofluorescence assay; western blot; polymerase chain reaction.