SciELO - Scientific Electronic Library Online

vol.56 número2Maturação e expulsão placentária em vacas das raças Holandesa e NeloreAnalgesia epidural com clonidina ou romifidina em cães submetidos à cirurgia coxofemoral índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

versão On-line ISSN 1678-4162


GEBARA, C.M.S. et al. Histopathological lesions in the central nervous system of dogs with encephalitis and molecular diagnosis of canine distemper virus infection. Arq. Bras. Med. Vet. Zootec. [online]. 2004, vol.56, n.2, pp.168-174. ISSN 1678-4162.

The reverse transcription-polymerase chain reaction (RT-PCR) was used to detect canine distemper virus (CDV) RNA in 10 urine samples from dogs that had died with clinical systemic and neurological signs indicative of CDV infection. Brain, cerebellum and urinary bladder fragments were collected for histopathological examination. For the negative control, urine and organ fragments were collected at necropsy from four dogs without clinical symptoms of infectious diseases that had died from other causes. The dogs positives in RT-PCR (9/10) presented histological lesions in the brain and cerebellum characteristic of acute (5/9) or chronic (4/9) encephalitis compatible with those caused by CDV. One of the dogs with neurological signs similar to those observed in canine distemper was negative in RT-PCR and presented non-specific histopathological alterations that could be associated to others nervous system diseases. Although the RT-PCR detected the CDV in urine of nine dogs with clinical signs of distemper, no histopathological alterations were observed in any of the urinary bladder samples. All the biological samples from the control dogs were negative in RT-PCR and did not present any histopathological alterations in organ. Besides describing the most characteristic histopathological lesion found in the central nervous system of dogs with acute and chronic encephalitis due to canine distemper, this study also showed the specificity of the RT-PCR technique in early and ante mortem diagnosis of CDV infections.

Palavras-chave : dog; canine distemper; canine distemper virus; RT-PCR; histopathology.

        · resumo em Português     · texto em Português     · Português ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons